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KMID : 1007520110200061713
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Food Science and Biotechnology
2011 Volume.20 No. 6 p.1713 ~ p.1719
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Anti-inflammatory Effect of Grape Seed may Involve the Induction of Heme Oxygenase-1 and Suppression of Nuclear Factor-κB Activation
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Sung Jee-Hye
Kim Young-Hwa
Choi Young-Min
Ham Hyeon-mi
Jeong Heon-Sang
Lee Jun-Soo
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Abstract
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This study aimed to elucidate the antiinflammatory mechanism of grape-seed acetone extract (GSAE) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The GSAE at the highest concentration (500 μg/mL) significantly inhibited nitric oxide production by approximately 78% and suppressed inducible nitric oxide synthase protein expression by approximately 83% compared to LPS-stimulated cells. The GSAE also suppressed inhibitor κBα phosphorylation and nuclear factor-κB (NF- κB; p65) transcriptional activity and translocation to the nucleus. In addition, GSAE induced the expression of heme oxygenase-1 (HO-1) in a dose-dependent manner, and that blocking HO-1 activity eliminated the inhibitory effects of the GSAE. The addition of carbon monoxide, a byproduct of heme degradation by HO-1, mimicked the inhibitory action of low concentrations of GSAE. These data suggest that GSAE carries out anti-inflammatory actions in macrophages through the induction of HO-1 and suppression of NF-κB activation.
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KEYWORD
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grape seed, anti-inflammation, nitric oxide, heme oxygenase-1, nuclear factor-κB
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